SPI1 antibody

Catalog No. ABIN2666294

The Mouse Monoclonal anti-SPI1 antibody (Clone 7C6B05) (ABIN2666294) specifically detects SPI1 in BCA. The antibody is reactive with Human samples.

Quick Overview for SPI1 antibody (ABIN2666294)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Biochemical Assay (BCA)

Clone: 7C6B05

Product Details anti-SPI1 Antibody

Purification: Affinity purified.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store undiluted between 2°C and 8°C.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1

Background: SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.

Pathways: Stem Cell Maintenance