AGO1 antibody (N-Term)

Catalog No. ABIN2668226

Cited in 2+ publications. The Rat Monoclonal anti-AGO1 antibody (Clone 4B8) (ABIN2668226) specifically detects AGO1 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for AGO1 antibody (N-Term) (ABIN2668226)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 4B8

Product Details anti-AGO1 Antibody

Binding Specificity: N-Term

Purpose: Ago1 antibody (mAb) (Clone 4B8)

Purification: Protein G Chromatography

Immunogen: This Ago1 antibody was raised against a peptide from the N-terminus of human Argonaute 1 (Ago1) protein.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-AGO1 antibody (ABIN2668226)

Beitzinger, Peters, Zhu, Kremmer, Meister: "Identification of human microRNA targets from isolated argonaute protein complexes." in: RNA biology, Vol. 4, Issue 2, pp. 76-84, (2007) (PubMed).

Höck, Weinmann, Ender, Rüdel, Kremmer, Raabe, Urlaub, Meister: "Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells." in: EMBO reports, Vol. 8, Issue 11, pp. 1052-60, (2007) (PubMed).

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: Ago1

Background: The Argonaute family proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain.

Molecular Weight: 100 kDa

Gene ID: 26523

NCBI Accession: NP_036331

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization