Nucleolin antibody

Catalog No. ABIN2668241

The Mouse Monoclonal anti-Nucleolin antibody (Clone 3G4B20) (ABIN2668241) specifically detects Nucleolin in WB, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Nucleolin antibody (ABIN2668241)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3G4B20

Product Details anti-Nucleolin Antibody

Purpose: Nucleolin antibody (mAb) (Clone 3G4B20)

Purification: Culture Supernatant

Immunogen: This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.

Isotype: IgG1

Application Details

Application Notes: ICC/IF: 1:500 dilution WB*: 1:500 - 1:2,000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91231. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Concentrated culture supernatant containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

Molecular Weight: 100 kDa

Gene ID: 4691

NCBI Accession: XP_850477

Pathways: Ribonucleoprotein Complex Subunit Organization