DNMT3A antibody

Catalog No. ABIN2668262

Cited in 3+ publications. The Mouse Monoclonal anti-DNMT3A antibody (Clone 64B1446) (ABIN2668262) specifically detects DNMT3A in WB, IHC and ChIP. The antibody is reactive with Human and Mouse samples.

Quick Overview for DNMT3A antibody (ABIN2668262)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Clone: 64B1446

Product Details anti-DNMT3A Antibody

Purpose: DNMT3A antibody (mAb) (Clone 64B1446)

Purification: Protein G Chromatography

Immunogen: This DNMT3A antibody was raised against a full-length protein corresponding to mouse DNMT3A.

Isotype: IgG1 kappa

Application Details

Application Notes: Validated Applications: WB: 2 - 4 μg/mL dilution Individual optimization may be required. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. Published Applications: ChIP IHC See references for more information.

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: 4 °C,-20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-DNMT3A antibody (ABIN2668262)

Petell, Alabdi, He, San Miguel, Rose, Gowher: "An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation." in: Nucleic acids research, Vol. 44, Issue 16, pp. 7605-17, (2016) (PubMed).

Karius, Schnekenburger, Ghelfi, Walter, Dicato, Diederich: "Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines." in: Biochemical pharmacology, Vol. 81, Issue 11, pp. 1329-42, (2011) (PubMed).

Saito, Kato, Hiraoka, Horii, Suzuki, Higashi, Kaji, Kondo, Yamamoto: "DNA methylation of colon mucosa in ulcerative colitis patients: correlation with inflammatory status." in: Inflammatory bowel diseases, Vol. 17, Issue 9, pp. 1955-65, (2011) (PubMed).

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A

Background: DNMT3A - DNA methyltransferase proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells.

Molecular Weight: 116 kDa

Gene ID: 1788

NCBI Accession: NP_001258682