Retinoblastoma Binding Protein 8 antibody (AA 620-897)

Catalog No. ABIN2668263

This Mouse Monoclonal antibody specifically detects Retinoblastoma Binding Protein 8 in WB, IP, ICC and IF. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for Retinoblastoma Binding Protein 8 antibody (AA 620-897) (ABIN2668263)

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma Binding Protein 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 14-1

Product Details anti-Retinoblastoma Binding Protein 8 Antibody

Binding Specificity: AA 620-897

Purification: Protein A Chromatography

Immunogen: This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Retinoblastoma Binding Protein 8 antibody (ABIN2668263)

Onyango, Howard, Neherin, Yanez, Stark: "Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination." in: Nucleic acids research, Vol. 44, Issue 12, pp. 5702-16, (2016) (PubMed).

Bakr, Köcher, Volquardsen, Petersen, Borgmann, Dikomey, Rothkamm, Mansour: "Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1." in: Oncotarget, Vol. 7, Issue 36, pp. 57679-57693, (2016) (PubMed).

Zhou, Caron, Legube, Paull: "Quantitation of DNA double-strand break resection intermediates in human cells." in: Nucleic acids research, Vol. 42, Issue 3, pp. e19, (2014) (PubMed).

Makharashvili, Tubbs, Yang, Wang, Barton, Zhou, Deshpande, Lee, Lobrich, Sleckman, Wu, Paull: "Catalytic and noncatalytic roles of the CtIP endonuclease in double-strand break end resection." in: Molecular cell, Vol. 54, Issue 6, pp. 1022-33, (2014) (PubMed).

Zhang, Jasin: "An essential role for CtIP in chromosomal translocation formation through an alternative end-joining pathway." in: Nature structural & molecular biology, Vol. 18, Issue 1, pp. 80-4, (2011) (PubMed).

Yu, Fu, Lai, Baer, Chen: "BRCA1 ubiquitinates its phosphorylation-dependent binding partner CtIP." in: Genes & development, Vol. 20, Issue 13, pp. 1721-6, (2006) (PubMed).

Target Details for Retinoblastoma Binding Protein 8

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Alternative Name: CtIP

Molecular Weight: 120 kDa

Gene ID: 5932

Pathways: Cell Division Cycle, DNA Damage Repair