SIRT1 antibody (AA 581-630)

Catalog No. ABIN2668278

This Mouse Monoclonal antibody specifically detects SIRT1 in WB, IP, IF and ICC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for SIRT1 antibody (AA 581-630) (ABIN2668278)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 2G1-F7

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 581-630

Purification: Protein G Chromatography

Immunogen: This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-SIRT1 antibody (ABIN2668278)

Rajendran, Kidane, Yu, Dashwood, Bisson, Löhr, Ho, Williams, Dashwood: "HDAC turnover, CtIP acetylation and dysregulated DNA damage signaling in colon cancer cells treated with sulforaphane and related dietary isothiocyanates." in: Epigenetics, Vol. 8, Issue 6, pp. 612-23, (2013) (PubMed).

Vaquero, Scher, Lee, Erdjument-Bromage, Tempst, Reinberg: "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin." in: Molecular cell, Vol. 16, Issue 1, pp. 93-105, (2004) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Molecular Weight: 120 kDa

Gene ID: 23411

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling