Histone H2A antibody (H2AS129ph)

Catalog No. ABIN2668303

There is 1 publication for this product available. The Rabbit Polyclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Saccharomyces cerevisiae. It has been validated for WB, DB and ChIP.

Quick Overview for Histone H2A antibody (H2AS129ph) (ABIN2668303)

Target: Histone H2A

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-Histone H2A Antibody

Binding Specificity: H2AS129ph

Purpose: Histone H2AS129ph antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H2A phospho Ser129 antibody was raised against a peptide including phospho-serine 129 of budding yeast Histone H2A.

Application Details

Application Notes: WB: 1:500 - 1:2,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H2A antibody (ABIN2668303)

Jha, Strahl: "An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair." in: Nature communications, Vol. 5, pp. 3965, (2014) (PubMed).

Target Details for Histone H2A

Target: Histone H2A

Background: Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2A variant, H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2A at serine 129 is required for the efficient repair of DNA double-strand breaks.

Molecular Weight: 14 kDa

NCBI Accession: NP_010511