PHC1 antibody

Catalog No. ABIN2668352

Cited in 2+ publications. The Mouse Monoclonal anti-PHC1 antibody (Clone 6-1-3) (ABIN2668352) specifically detects PHC1 in WB, IF, IP and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for PHC1 antibody (ABIN2668352)

Target: PHC1 (Polyhomeotic-Like 1 (PHC1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PHC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 6-1-3

Product Details anti-PHC1 Antibody

Purpose: Phc1 antibody (mAb) (Clone 6-1-3)

Purification: Protein G Chromatography

Immunogen: This Phc1 antibody was raised against a recombinant mouse Phc1 protein fragment.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-PHC1 antibody (ABIN2668352)

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." in: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Miyagishima, Isono, Fujimura, Iyo, Takihara, Masumoto, Vidal, Koseki: "Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase." in: Histochemistry and cell biology, Vol. 120, Issue 2, pp. 111-9, (2003) (PubMed).

Target Details for PHC1

Target: PHC1 (Polyhomeotic-Like 1 (PHC1))

Alternative Name: Phc1

Background: Phc1, polyhomeotic homolog 1 (also known as EDR1, early development regulatory protein 1) is a component of the polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is required to maintain the stable repression of the Hox genes during development. This chromatin-associated protein complex acts via chromatin remodeling and histone modifications. Phc1 has been shown to interact with PHC2, BMI1, MAPKAPK2 and MCRS1.

Molecular Weight: 150 kDa

Gene ID: 1911

NCBI Accession: NP_001036088