SIN3A antibody (N-Term)

Catalog No. ABIN2668357

There is 1 publication for this product available. The Rabbit Polyclonal anti-SIN3A antibody is suitable to detect SIN3A in samples from Human and Mouse. It has been validated for WB and ChIP.

Quick Overview for SIN3A antibody (N-Term) (ABIN2668357)

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3A antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-SIN3A Antibody

Binding Specificity: N-Term

Purpose: SIN3A antibody (pAb)

Purification: Unpurified

Immunogen: This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.

Application Details

Application Notes: ChIP: 5 - 10 µL per ChIP WB*: 1:1,000 - 1:5,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SIN3A antibody (ABIN2668357)

Chalei, Sansom, Kong, Lee, Montiel, Vance, Ponting: "The long non-coding RNA Dali is an epigenetic regulator of neural differentiation." in: eLife, Vol. 3, pp. e04530, (2014) (PubMed).

Target Details for SIN3A

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Alternative Name: SIN3A

Background: SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.

Molecular Weight: 155 kDa

Gene ID: 25942

NCBI Accession: NP_001138829

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha