ATAD2 antibody (C-Term)

Catalog No. ABIN2668366

The Rabbit Polyclonal anti-ATAD2 antibody is suitable to detect ATAD2 in samples from Human. It has been validated for WB and IP.

Quick Overview for ATAD2 antibody (C-Term) (ABIN2668366)

Target: ATAD2 (ATPase Family, AAA Domain Containing 2 (ATAD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ATAD2 Antibody

Binding Specificity: C-Term

Purpose: ATAD2 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human ATAD2.

Isotype: IgG

Application Details

Application Notes: IP: 5 - 10 µL per IP WB*: 1:500 - 1:2,000 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for ATAD2

Target: ATAD2 (ATPase Family, AAA Domain Containing 2 (ATAD2))

Alternative Name: ATAD2

Background: ATAD2 (ATPase family AAA domain-containing protein 2) may be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.

Molecular Weight: 160 kDa

Gene ID: 29028

NCBI Accession: NP_054828