SIP1 antibody (AA 1-360)

Catalog No. ABIN2668496

Cited in 3+ publications. The Mouse Monoclonal anti-SIP1 antibody (Clone 6E5) (ABIN2668496) specifically detects SIP1 in WB, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for SIP1 antibody (AA 1-360) (ABIN2668496)

Target: SIP1 (GEMIN2) (Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 6E5

Product Details anti-SIP1 Antibody

Binding Specificity: AA 1-360

Purpose: SIP1 antibody (mAb) (Clone 6E5)

Purification: Protein G Chromatography

Immunogen: This SIP1 antibody was raised against a recombinant protein corresponding to amino acids 1-360 of human SIP1.

Isotype: IgG2a

Application Details

Application Notes: WB: 1 - 4 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SIP1 antibody (ABIN2668496)

Wiles, Bell, Thomas, Beckerle, Lessnick: "ZEB2 Represses the Epithelial Phenotype and Facilitates Metastasis in Ewing Sarcoma." in: Genes & cancer, Vol. 4, Issue 11-12, pp. 486-500, (2014) (PubMed).

Tange, Oktyabri, Terashima, Ishimura, Suzuki: "JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines." in: PLoS ONE, Vol. 9, Issue 12, pp. e115684, (2014) (PubMed).

Oztas, Avci, Ozcan, Sayan, Tulchinsky, Yagci: "Novel monoclonal antibodies detect Smad-interacting protein 1 (SIP1) in the cytoplasm of human cells from multiple tumor tissue arrays." in: Experimental and molecular pathology, Vol. 89, Issue 2, pp. 182-9, (2010) (PubMed).

Target Details for SIP1

Target: SIP1 (GEMIN2) (Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2))

Alternative Name: SIP1

Background: SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).

Molecular Weight: 180 kDa

Gene ID: 8487

NCBI Accession: NP_055610

Pathways: Ribonucleoprotein Complex Subunit Organization, Tube Formation