TET3 antibody (AA 1159-1329)

Catalog No. ABIN2668513

There are 2+ publications for this product available. The Rabbit Polyclonal anti-TET3 antibody is suitable to detect TET3 in samples from Mouse. It has been validated for WB and ChIP.

Quick Overview for TET3 antibody (AA 1159-1329) (ABIN2668513)

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TET3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-TET3 Antibody

Binding Specificity: AA 1159-1329

Purpose: Tet3 antibody (pAb)

Purification: Unpurified

Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 1159-1329 of mouse Tet3.

Application Details

Application Notes: WB: 1:1,000 - 1:5,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-TET3 antibody (ABIN2668513)

Thienpont, Steinbacher, Zhao, DAnna, Kuchnio, Ploumakis, Ghesquière, Van Dyck, Boeckx, Schoonjans, Hermans, Amant, Kristensen, Koh, Mazzone, Coleman, Carell, Carmeliet, Lambrechts: "Tumour hypoxia causes DNA hypermethylation by reducing TET activity." in: Nature, Vol. 537, Issue 7618, pp. 63-68, (2016) (PubMed).

Otani, Kimura, Sharif, Endo, Mishima, Kawakami, Koseki, Shirakawa, Suetake, Tajima: "Cell cycle-dependent turnover of 5-hydroxymethyl cytosine in mouse embryonic stem cells." in: PLoS ONE, Vol. 8, Issue 12, pp. e82961, (2013) (PubMed).

Target Details for TET3

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Alternative Name: Tet3

Background: Tet3 (tet methylcytosine dioxygenase 3) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC.

Molecular Weight: 200 kDa

Gene ID: 194388

NCBI Accession: NP_898961

Pathways: Warburg Effect