TWIST1 antibody

Catalog No. ABIN2668516

There are 5+ publications for this product available. The Mouse Monoclonal anti-TWIST1 antibody is suitable to detect TWIST1 in samples from Human and Mouse. It has been validated for WB, IHC, ICC and IF.

Quick Overview for TWIST1 antibody (ABIN2668516)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TWIST1 Antibody

Purpose: TWIST antibody (mAb)

Purification: Protein G Chromatography

Immunogen: This TWIST antibody was raised against a recombinant protein corresponding to full-length mouse TWIST.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-TWIST1 antibody (ABIN2668516)

Ezponda, Popovic, Shah, Martinez-Garcia, Zheng, Min, Will, Neri, Kelleher, Yu, Licht: "The histone methyltransferase MMSET/WHSC1 activates TWIST1 to promote an epithelial-mesenchymal transition and invasive properties of prostate cancer." in: Oncogene, Vol. 32, Issue 23, pp. 2882-90, (2013) (PubMed).

Casas, Kim, Bendesky, Ohno-Machado, Wolfe, Yang: "Snail2 is an essential mediator of Twist1-induced epithelial mesenchymal transition and metastasis." in: Cancer research, Vol. 71, Issue 1, pp. 245-54, (2011) (PubMed).

Eckert, Lwin, Chang, Kim, Danis, Ohno-Machado, Yang: "Twist1-induced invadopodia formation promotes tumor metastasis." in: Cancer cell, Vol. 19, Issue 3, pp. 372-86, (2011) (PubMed).

Kida, Asahina, Teraoka, Gitelman, Sato: "Twist relates to tubular epithelial-mesenchymal transition and interstitial fibrogenesis in the obstructed kidney." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 55, Issue 7, pp. 661-73, (2007) (PubMed).

Gitelman: "Twist protein in mouse embryogenesis." in: Developmental biology, Vol. 189, Issue 2, pp. 205-14, (1997) (PubMed).

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST

Background: TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial-mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.

Molecular Weight: 21 kDa

Gene ID: 7291

NCBI Accession: NP_000465

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity