CHD4 antibody (AA 1641-1662)

Catalog No. ABIN2668526

Cited in 1 publication. The Rat Monoclonal anti-CHD4 antibody (Clone 4D8) (ABIN2668526) specifically detects CHD4 in WB, IF and IP. The antibody is reactive with Drosophila melanogaster samples.

Quick Overview for CHD4 antibody (AA 1641-1662) (ABIN2668526)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reactivity: Drosophila melanogaster

Host: Rat

Clonality: Monoclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 4D8

Product Details anti-CHD4 Antibody

Binding Specificity: AA 1641-1662

Purpose: Mi-2 antibody (mAb) (Clone 4D8)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acid residues 1641-1662 of Drosophila Mi-2.

Isotype: IgG2c

Application Details

Application Notes: For Mi-2 beta (CHD4), we also offer AbFlex Mi-2 beta (CHD4) Recombinant Antibody (rAb). For details, see Catalog No. 91275.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-CHD4 antibody (ABIN2668526)

Murawska, Kunert, van Vugt, Längst, Kremmer, Logie, Brehm: "dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2745-57, (2008) (PubMed).

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: Mi-2

Background: Mi-2 (ATP-dependent helicase Mi-2) is a member of the CHD family of ATP-dependent chromatin remodelers characterized by the presence of two tandemly arranged chromodomains. The Chd3/Chd4 subfamily contains a pair of PHD fingers preceding the chromodomains and these proteins exist in multisubunit complexes with nucleosome remodeling and deacetylation (NuRD) activity. Drosophila Mi-2 (dMI-2) is the orthologs of mammalian CHD4.

Molecular Weight: 220 kDa

Gene ID: 40170

NCBI Accession: NP_649154