MAD2L1 antibody

Catalog No. ABIN2668530

This Mouse Monoclonal antibody specifically detects MAD2L1 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for MAD2L1 antibody (ABIN2668530)

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 17D10

Product Details anti-MAD2L1 Antibody

Purpose: MAD2L1 antibody (mAb) (Clone 17D10)

Purification: Protein G Chromatography

Immunogen: This MAD2L1 antibody was raised against full-length recombinant human MAD2L1.

Isotype: IgG1

Application Details

Application Notes: WB: 2 - 5 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: MAD2L1

Background: MAD2L1 (aka HSMAD2, MAD2, MAD2-like 1) is the human homolog of the budding yeast MAD2, or mitotic arrest deficient 2 gene. MAD2L1 is a component of the mitotic spindle assembly checkpoint, which monitors the attachment of the mitotic spindle to the kinetochores of segregating chromosomes and delays the onset of anaphase until attachment is complete and the chromosomes are properly aligned on the metaphase plate. MAD2L1 associates only with unattached kinetochores in prometaphase, and prevents cell cycle progression by associating with the Anaphase Promoting Complex (APC), specifically restricting the activity of the CDC20 protein.

Molecular Weight: 23 kDa

Gene ID: 4085

NCBI Accession: NP_002349