POLR2A/RPB1 antibody

Catalog No. ABIN2668535

The Rat Monoclonal anti-POLR2A/RPB1 antibody (Clone 1F4B6) (ABIN2668535) specifically detects POLR2A/RPB1 in WB, IF, ICC, ChIP and ChIP-seq. The antibody is reactive with Human samples.

Quick Overview for POLR2A/RPB1 antibody (ABIN2668535)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1F4B6

Product Details anti-POLR2A/RPB1 Antibody

Purpose: RNA pol II antibody (mAb) (Clone 1F4B6)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.

Isotype: IgG2a

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 5 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. For RNA Pol II, we also offer AbFlex RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A / RNA Polymerase II

Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight: 240 kDa

Gene ID: 5430

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways