Histone H2B antibody (Lys120, ubLys120)

Catalog No. ABIN2668551

This Mouse Monoclonal antibody specifically detects Histone H2B in WB and ChIP. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for Histone H2B antibody (Lys120, ubLys120) (ABIN2668551)

Target: Histone H2B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 56

Product Details anti-Histone H2B Antibody

Binding Specificity: Lys120, ubLys120

Purification: Protein G Chromatography

Immunogen: This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone H2B antibody (ABIN2668551)

Materne, Vázquez, Sánchez, Yague-Sanz, Anandhakumar, Migeot, Antequera, Hermand: "Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus." in: eLife, Vol. 5, (2016) (PubMed).

Mbogning, Nagy, Pagé, Schwer, Shuman, Fisher, Tanny: "The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast." in: PLoS genetics, Vol. 9, Issue 12, pp. e1004029, (2014) (PubMed).

DeGennaro, Alver, Marguerat, Stepanova, Davis, Bähler, Park, Winston: "Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast." in: Molecular and cellular biology, Vol. 33, Issue 24, pp. 4779-92, (2013) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Molecular Weight: 26 kDa

Gene ID: 8348