CENPE antibody

Catalog No. ABIN2668581

This Mouse Monoclonal antibody specifically detects CENPE in ICC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CENPE antibody (ABIN2668581)

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPE antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1H12

Product Details anti-CENPE Antibody

Purpose: CENP-E antibody (mAb) (Clone 1H12)

Purification: Protein G Chromatography

Immunogen: This CENP-E antibody was raised against full-length human CENP-E protein.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1 μg/mL dilution This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Alternative Name: CENP-E

Background: Centrosome-associated protein E (CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase.

Molecular Weight: 320 kDa

NCBI Accession: NP_001804

Pathways: M Phase, Maintenance of Protein Location