SOX2 antibody (AA 111-140)

Catalog No. ABIN2668593

This Rabbit Polyclonal antibody specifically detects SOX2 in WB, IHC, IF and ICC. It exhibits reactivity toward Mouse. It has been mentioned in 6+ publications

Quick Overview for SOX2 antibody (AA 111-140) (ABIN2668593)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SOX2 Antibody

Binding Specificity: AA 111-140

Purpose: Sox2 antibody (pAb)

Purification: Unpurified

Immunogen: This Sox2 antibody was raised against a peptide corresponding to amino acids amino acids 111-140 mouse Sox2.

Application Details

Application Notes: WB: 1:2,000 - 1:10,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SOX2 antibody (ABIN2668593)

Jones, Han, DeBruyne, Philpot: "Persistent neuronal Ube3a expression in the suprachiasmatic nucleus of Angelman syndrome model mice." in: Scientific reports, Vol. 6, pp. 28238, (2016) (PubMed).

Lee, Chen, Dhar, Gu, Chen, Kim, Li, Lee: "A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, Nanog, Klf4 and c-Myc to regulate stemness." in: Nucleic acids research, Vol. 44, Issue 22, pp. 10603-10618, (2016) (PubMed).

Drakulic, Vicentic, Schwirtlich, Tosic, Krstic, Klajn, Stevanovic: "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1." in: Anais da Academia Brasileira de Ciências, Vol. 87, Issue 1, pp. 389-404, (2015) (PubMed).

Man, Wells, Carter: "Cellular distribution of Egr1 transcription in the male rat pituitary gland." in: Journal of molecular endocrinology, Vol. 53, Issue 2, pp. 271-80, (2014) (PubMed).

Seo, Basu-Roy, Zavadil, Basilico, Mansukhani: "Distinct functions of Sox2 control self-renewal and differentiation in the osteoblast lineage." in: Molecular and cellular biology, Vol. 31, Issue 22, pp. 4593-608, (2011) (PubMed).

Tompkins, Besnard, Lange, Wert, Keiser, Smith, Lang, Whitsett: "Sox2 is required for maintenance and differentiation of bronchiolar Clara, ciliated, and goblet cells." in: PLoS ONE, Vol. 4, Issue 12, pp. e8248, (2009) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: Sox2

Background: Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct-4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct-4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3.

Molecular Weight: 35 kDa

Gene ID: 6657

NCBI Accession: NP_035573

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis