SIRT2 antibody (pSer331)

Catalog No. ABIN2668611

This Rat Monoclonal antibody specifically detects SIRT2 in WB. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for SIRT2 antibody (pSer331) (ABIN2668611)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6B5

Product Details anti-SIRT2 Antibody

Binding Specificity: pSer331

Purpose: SIRT2 phospho Ser331 antibody (mAb) (Clone 6B5)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide corresponding to human SIRT2 phosphorylated at Serine 331.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SIRT2 antibody (ABIN2668611)

Pandithage, Lilischkis, Harting, Wolf, Jedamzik, Lüscher-Firzlaff, Vervoorts, Lasonder, Kremmer, Knöll, Lüscher: "The regulation of SIRT2 function by cyclin-dependent kinases affects cell motility." in: The Journal of cell biology, Vol. 180, Issue 5, pp. 915-29, (2008) (PubMed).

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Background: SIRT2 (sirtuin 2) is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARg and subsequent repression of PPARg mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis. Ser-331 is phosphorylated by the cyclin kinases Cdk2 and Cdk5 and this phosphorylation inhibits SIRT2's catalytic activity.

Molecular Weight: 40 & 43 kDa

Gene ID: 22933

NCBI Accession: NP_036369