BMI1 antibody

Catalog No. ABIN2668619

Cited in 2+ publications. The Mouse Monoclonal anti-BMI1 antibody (Clone AF27) (ABIN2668619) specifically detects BMI1 in WB, IHC, IF, ChIP-seq and ChIP. The antibody is reactive with Human and Mouse samples.

Quick Overview for BMI1 antibody (ABIN2668619)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Clone: AF27

Product Details anti-BMI1 Antibody

Purpose: BMI-1 antibody (mAb) (Clone AF27)

Purification: Protein G Chromatography

Immunogen: This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.

Isotype: IgG2a

Application Details

Application Notes: ChIP-Seq: 4 μg per ChIP WB: 0.5 - 2 μg/mL For optimal results, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-BMI1 antibody (ABIN2668619)

Jin, Lee, Heo, Lim, Kim, Kim, Nam, Hong, Cho, Choi, Kim, Shin, Kim: "Senescence associated MCP-1 secretion is dependent on a decline in BMI1 in human mesenchymal stromal cells." in: Antioxidants & redox signaling, (2015) (PubMed).

Lafkas, Rodilla, Huyghe, Mourao, Kiaris, Fre: "Notch3 marks clonogenic mammary luminal progenitor cells in vivo." in: The Journal of cell biology, Vol. 203, Issue 1, pp. 47-56, (2013) (PubMed).

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI-1

Background: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

Molecular Weight: 40 kDa

Gene ID: 6605

NCBI Accession: NP_031578

Pathways: Cell Division Cycle, Autophagy