BMI1 antibody

Catalog No. ABIN2668620

This Mouse Monoclonal antibody specifically detects BMI1 in WB, IF and ICC. It exhibits reactivity toward Human and Mouse.It has been mentioned in 1 publication

Quick Overview for BMI1 antibody (ABIN2668620)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: DC9

Product Details anti-BMI1 Antibody

Purpose: BMI-1 antibody (mAb) (Clone DC9)

Purification: Protein G Chromatography

Immunogen: This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.

Isotype: IgG1 kappa

Application Details

Application Notes: ICC/IF: 0.5 μg/mL dilution For ChIP-Seq analysis of BMI-1, we offer AbFlex BMI-1 Recombinant Antibody (rAb). For details, see Catalog No. 91195.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-BMI1 antibody (ABIN2668620)

Bracken, Kleine-Kohlbrecher, Dietrich, Pasini, Gargiulo, Beekman, Theilgaard-Mönch, Minucci, Porse, Marine, Hansen, Helin: "The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells." in: Genes & development, Vol. 21, Issue 5, pp. 525-30, (2007) (PubMed).

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI-1

Background: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

Molecular Weight: 40 kDa

Gene ID: 648

NCBI Accession: NP_031578

Pathways: Cell Division Cycle, Autophagy