MITF antibody (N-Term)

Catalog No. ABIN2668714

There are 3+ publications for this product available. The Mouse Monoclonal anti-MITF antibody is suitable to detect MITF in samples from Human. It has been validated for WB, IF, ICC, IP, ChIP-seq, ChIP and SSA.

Quick Overview for MITF antibody (N-Term) (ABIN2668714)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Supershift Assay (SSA)

Clone: C5-D5

Product Details anti-MITF Antibody

Binding Specificity: N-Term

Purpose: MITF antibody (mAb) (Clone C5.D5)

Purification: Protein G Chromatography

Immunogen: This MITF antibody was raised against an N-terminal fragment of human MITF.

Isotype: IgG1

Application Details

Application Notes: ChIP: 2.5 - 10 μg per ChIP ChIP-Seq: 4 μg each This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91201.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-MITF antibody (ABIN2668714)

Wellbrock, Marais: "Elevated expression of MITF counteracts B-RAF-stimulated melanocyte and melanoma cell proliferation." in: The Journal of cell biology, Vol. 170, Issue 5, pp. 703-8, (2005) (PubMed).

Du, Miller, Widlund, Horstmann, Ramaswamy, Fisher: "MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma." in: The American journal of pathology, Vol. 163, Issue 1, pp. 333-43, (2003) (PubMed).

Hemesath, Price, Takemoto, Badalian, Fisher: "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes." in: Nature, Vol. 391, Issue 6664, pp. 298-301, (1998) (PubMed).

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.

Molecular Weight: 52-56 kDa

Gene ID: 4286

NCBI Accession: NP_937802

Pathways: Chromatin Binding