EED antibody

Catalog No. ABIN2668749

This Mouse Monoclonal antibody specifically detects EED in WB, IHC, ChIP-seq and ChIP. It exhibits reactivity toward Human.

Quick Overview for EED antibody (ABIN2668749)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Clone: 41D

Product Details anti-EED Antibody

Purpose: EED antibody (mAb) (Clone 41D)

Purification: Protein A Chromatography

Immunogen: This EED antibody was raised against full-length recombinant human EED protein.

Isotype: IgG2a

Application Details

Application Notes: ChIP: 4 µL per ChIP ChIP-Seq: 4 µL each IHC: 2 - 8 μg/mL dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91135.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED

Background: EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression.

Molecular Weight: 57 kDa

Gene ID: 8726

NCBI Accession: NP_003788