MECP2 antibody (pSer80)

Catalog No. ABIN2668830

This Rabbit Polyclonal antibody specifically detects MECP2 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for MECP2 antibody (pSer80) (ABIN2668830)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MECP2 Antibody

Binding Specificity: pSer80

Purpose: MeCP2 phospho Ser80 antibody (pAb)

Purification: Unpurified

Immunogen: This MeCP2 phospho Ser80 antibody was raised against a peptide containing phospho Ser80 of mouse MeCP2.

Application Details

Application Notes: WB: 1:2,000 - 1:10,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2

Background: Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl-CpG binding protein 2)can repress transcription by recruiting the Sin3 complex including histone deacetylases (HDACs). MeCP2 is distributed throughout the nucleus in human cells. Phosphorylation of MeCP2 at serine 80 may regulate the activity of neurons switching between resting and active states in neural circuits.

Molecular Weight: 70 kDa

Gene ID: 4204

NCBI Accession: NP_001075448

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane