Lamin A/C antibody (C-Term, Mature)

Catalog No. ABIN2668842

This Mouse Monoclonal antibody specifically detects Lamin A/C in WB, IF, ICC and IP. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Lamin A/C antibody (C-Term, Mature) (ABIN2668842)

Target: Lamin A/C (LMNA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 4A4

Product Details anti-Lamin A/C Antibody

Binding Specificity: C-Term, Mature

Purpose: Lamin A, mature antibody (mAb) (Clone 4A4)

Purification: Protein G Chromatography

Immunogen: This Lamin A, mature antibody was raised against a peptide within the C-terminus of mature human Lamin A.

Isotype: IgG1

Application Details

Application Notes: WB*: 0.05 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Lamin A/C antibody (ABIN2668842)

Roblek, Schüchner, Huber, Ollram, Vlcek-Vesely, Foisner, Wehnert, Ogris: "Monoclonal antibodies specific for disease-associated point-mutants: lamin A/C R453W and R482W." in: PLoS ONE, Vol. 5, Issue 5, pp. e10604, (2010) (PubMed).

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A

Background: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Molecular Weight: 74 kDa

Gene ID: 4000

NCBI Accession: NP_733821

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus