Lamin A/C antibody (AA 430-545)

Catalog No. ABIN7938325

Cited in 3+ publications. The Mouse Monoclonal anti-Lamin A/C antibody (Clone 3A6-4C11) (ABIN7938325) specifically detects Lamin A/C in WB, IF, ICC, IP and ChIP. The antibody is reactive with Human, Mouse, Rat, Monkey and Hamster samples.

Quick Overview for Lamin A/C antibody (AA 430-545) (ABIN7938325)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat, Monkey, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 3A6-4C11

Product Details anti-Lamin A/C Antibody

Binding Specificity: AA 430-545

Purpose: Lamin A/C antibody (mAb) (Clone 3A6-4C11)

Purification: Culture Supernatant

Immunogen: This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500 - 1:4000 dilution ICC/IF: 1:500 - 1:2000 dilution Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. For immunofluorescence experiments we recommend using paraformaldehyde (PFA) fixation. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91189.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Cell culture supernatant containing mouse IgG, 30 % glycerol, 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Lamin A/C antibody (ABIN7938325)

Gesson, Rescheneder, Skoruppa, von Haeseler, Dechat, Foisner: "A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2 alpha." in: Genome research, Vol. 26, Issue 4, pp. 462-73, (2016) (PubMed).

Kim, Zheng: "Generation and characterization of a conditional deletion allele for Lmna in mice." in: Biochemical and biophysical research communications, Vol. 440, Issue 1, pp. 8-13, (2013) (PubMed).

Eckersley-Maslin, Bergmann, Lazar, Spector: "Lamin A/C is expressed in pluripotent mouse embryonic stem cells." in: Nucleus (Austin, Tex.), Vol. 4, Issue 1, pp. 53-60, (2013) (PubMed).

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A/C

Background: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Molecular Weight: 75 kDa

Gene ID: 4000

NCBI Accession: NP_733821

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus