ASH2L antibody

Catalog No. ABIN2668850

Cited in 2+ publications. The Rabbit Polyclonal anti-ASH2L antibody (ABIN2668850) specifically detects ASH2L in WB, ChIP and ChIP-seq. The antibody is reactive with Human samples.

Quick Overview for ASH2L antibody (ABIN2668850)

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASH2L antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-ASH2L Antibody

Purpose: ASH2L antibody (pAb)

Purification: Unpurified

Immunogen: This ASH2L antibody was raised against full-length recombinant human ASH2L protein.

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB*: 1:500 - 1:2,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-ASH2L antibody (ABIN2668850)

Fossati, Dolfini, Donati, Mantovani: "NF-Y recruits Ash2L to impart H3K4 trimethylation on CCAAT promoters." in: PLoS ONE, Vol. 6, Issue 3, pp. e17220, (2011) (PubMed).

Cao, Chen, Cierpicki, Liu, Basrur, Lei, Dou: "An Ash2L/RbBP5 heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1 SET domain." in: PLoS ONE, Vol. 5, Issue 11, pp. e14102, (2010) (PubMed).

Target Details for ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Alternative Name: ASH2L

Background: ASH2L (absent, small or homeotic discs 2) is a trithorax protein and is a component of different protein complexes, including i.e. mixed-lineage leukemia (MLL) oncoproteins, which contain histone methyltransferase activity. These complexes trimethylate the lysine 4 of histone H3. Histone H3 trimethyl Lys4 is one of histone modifications that are part of elaborate mechanisms that evolved to control the structure of the chromatin.

Molecular Weight: 77 kDa

Gene ID: 430

NCBI Accession: NP_004665