WHSC1 antibody (AA 1-647)

Catalog No. ABIN2668917

This Mouse Monoclonal antibody specifically detects WHSC1 in WB, IF and ICC. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for WHSC1 antibody (AA 1-647) (ABIN2668917)

Target: WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WHSC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 29D1

Product Details anti-WHSC1 Antibody

Binding Specificity: AA 1-647

Purpose: MMSET / WHSC1 antibody (mAb) (Clone 29D1)

Purification: Protein G Chromatography

Immunogen: This MMSET / WHSC1 antibody was raised against a recombinant protein corresponding to amino acids 1-647 of human MMSET-I.

Isotype: IgG2b

Application Details

Application Notes: ICC/IF: 0.5 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-WHSC1 antibody (ABIN2668917)

García-Carpizo, Sarmentero, Han, Graña, Ruiz-Llorente, Pisano, Serrano, Brooks, Campbell, Barrero: "NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation." in: Scientific reports, Vol. 6, pp. 32952, (2016) (PubMed).

Marango, Shimoyama, Nishio, Meyer, Min, Sirulnik, Martinez-Martinez, Chesi, Bergsagel, Zhou, Waxman, Leibovitch, Walsh, Licht: "The MMSET protein is a histone methyltransferase with characteristics of a transcriptional corepressor." in: Blood, Vol. 111, Issue 6, pp. 3145-54, (2008) (PubMed).

Target Details for WHSC1

Target: WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1))

Alternative Name: MMSET / WHSC1

Background: MMSET (Multiple Myeloma SET domain-containing protein) / WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1, also known as NSD2, Nuclear Receptor binding SET domain protein 2, TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The MMSET / WHSC1 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15 % of patients with Multiple Myeloma (MM), a chromosomal translocation leads to MMSET / WHSC1 gene being aberrantly placed under the control of the IgH intronic u enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in MMSET / WHSC1 expression in a myeloma cell line suppressed its growth, suggesting a role for MMSET in maintaining the transformed state.

Molecular Weight: 88 & 157 kDa

Gene ID: 7468

NCBI Accession: NP_001035889

Pathways: SARS-CoV-2 Protein Interactome