EZH2 antibody (AA 353-451)

Catalog No. ABIN7938124

Cited in 8+ publications. The Mouse Monoclonal anti-EZH2 antibody (Clone AC22) (ABIN7938124) specifically detects EZH2 in ChIP. The antibody is reactive with Human and Mouse samples.

Quick Overview for EZH2 antibody (AA 353-451) (ABIN7938124)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Chromatin Immunoprecipitation (ChIP)

Clone: AC22

Product Details anti-EZH2 Antibody

Binding Specificity: AA 353-451

Purpose: EZH2 antibody (mAb) (Clone AC22)

Purification: Protein A Chromatography

Immunogen: This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.

Isotype: IgG2a

Application Details

Application Notes: ChIP: 1 - 3 μg per ChIP CUT&RUN: 1 µL per 50 µL reaction This antibody has been published, but not validated by Active Motif, for Western blot. For an antibody we have tested in use in Western blotting, see Catalog No. 39933. For EZH2, we also offer an AbFlex EZH2 Recombinant Antibody (rAb). For details, see Catalog No. 91159.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-EZH2 antibody (ABIN7938124)

Toth, Papp, Brulois, Choi, Gao, Jung: "LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection." in: PLoS pathogens, Vol. 12, Issue 9, pp. e1005878, (2016) (PubMed).

Ranzani, Rossetti, Panzeri, Arrigoni, Bonnal, Curti, Gruarin, Provasi, Sugliano, Marconi, De Francesco, Geginat, Bodega, Abrignani, Pagani: "The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4." in: Nature immunology, Vol. 16, Issue 3, pp. 318-325, (2015) (PubMed).

Bengani, Mendiratta, Maini, Vasanthi, Sultana, Ghasemi, Ahluwalia, Ramachandran, Mishra, Brahmachari: "Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome." in: PLoS ONE, Vol. 8, Issue 6, pp. e67217, (2013) (PubMed).

Wang, Pan, Lee, Martinez, Feng, Dong: "Cutting edge: Smad2 and Smad4 regulate TGF-β-mediated Il9 gene expression via EZH2 displacement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4908-12, (2013) (PubMed).

Rossetto, Tarrant-Elorza, Pari: "Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells." in: PLoS pathogens, Vol. 9, Issue 5, pp. e1003366, (2013) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." in: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Woellmer, Arteaga-Salas, Hammerschmidt: "BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression." in: PLoS pathogens, Vol. 8, Issue 9, pp. e1002902, (2012) (PubMed).

Murata, Kondo, Sugimoto, Kawashima, Saito, Isomura, Kanda, Tsurumi: "Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells." in: Journal of virology, Vol. 86, Issue 9, pp. 4752-61, (2012) (PubMed).

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background: EZH2 - (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Molecular Weight: 96 kDa

Gene ID: 2146

NCBI Accession: NP_004447

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation