5-Formylcytosine antibody

Catalog No. ABIN2668977

This Rabbit Polyclonal antibody specifically detects 5-Formylcytosine in DB, ICC, IF and WB. It has been mentioned in 1 publication

Quick Overview for 5-Formylcytosine antibody (ABIN2668977)

Target: 5-Formylcytosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Product Details

Purpose: 5-Formylcytosine (5-fC) antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.

Isotype: IgG

Application Details

Application Notes: DB: 0.5 - 2 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Target Details

Target: 5-Formylcytosine

Target Type: Chemical

Background: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.