5-Formylcytosine antibody

Catalog No. ABIN2668978

There are 4+ publications for this product available. The Rabbit Polyclonal anti-5-Formylcytosine antibody is suitable to detect 5-Formylcytosine. It has been validated for DB, ICC, IF, WB and IHC.

Quick Overview for 5-Formylcytosine antibody (ABIN2668978)

Target: 5-Formylcytosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Purpose: 5-Formylcytosine (5-fC) antibody (pAb)

Purification: Unpurified

Immunogen: This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.

Application Details

Application Notes: DB: 1:5,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References

Nettersheim, Heukamp, Fronhoffs, Grewe, Haas, Waha, Honecker, Waha, Kristiansen, Schorle: "Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development." in: PLoS ONE, Vol. 8, Issue 12, pp. e82881, (2014) (PubMed).

Müller, Bauer, Siegl, Rottach, Leonhardt: "TET-mediated oxidation of methylcytosine causes TDG or NEIL glycosylase dependent gene reactivation." in: Nucleic acids research, Vol. 42, Issue 13, pp. 8592-604, (2014) (PubMed).

Zhang, Szulwach, Hon, Song, Park, Yu, Lu, Dai, Wang, Street, Tan, Min, Ren, Jin, He: "Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing." in: Nature communications, Vol. 4, pp. 1517, (2013) (PubMed).

Inoue, Shen, Dai, He, Zhang: "Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development." in: Cell research, Vol. 21, Issue 12, pp. 1670-6, (2011) (PubMed).

Target Details

Target: 5-Formylcytosine

Target Type: Chemical

Background: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.