5-Formylcytosine antibody

Catalog No. ABIN2668978

There are 4+ publications for this product available. The Rabbit Polyclonal anti-5-Formylcytosine antibody is suitable to detect 5-Formylcytosine. It has been validated for DB, ICC, IF and IHC.

Quick Overview for 5-Formylcytosine antibody (ABIN2668978)

Target: 5-Formylcytosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details

Purification: None

Immunogen: This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References

Nettersheim, Heukamp, Fronhoffs, Grewe, Haas, Waha, Honecker, Waha, Kristiansen, Schorle: "Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development." in: PLoS ONE, Vol. 8, Issue 12, pp. e82881, (2014) (PubMed).

Müller, Bauer, Siegl, Rottach, Leonhardt: "TET-mediated oxidation of methylcytosine causes TDG or NEIL glycosylase dependent gene reactivation." in: Nucleic acids research, Vol. 42, Issue 13, pp. 8592-604, (2014) (PubMed).

Zhang, Szulwach, Hon, Song, Park, Yu, Lu, Dai, Wang, Street, Tan, Min, Ren, Jin, He: "Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing." in: Nature communications, Vol. 4, pp. 1517, (2013) (PubMed).

Inoue, Shen, Dai, He, Zhang: "Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development." in: Cell research, Vol. 21, Issue 12, pp. 1670-6, (2011) (PubMed).

Target Details

Target: 5-Formylcytosine

Target Type: Chemical