5-Methylcytosine (5MeC) antibody

Catalog No. ABIN2668982

Cited in 10+ publications. The Mouse Monoclonal anti-5-Methylcytosine antibody (Clone 33D3) (ABIN2668982) specifically detects 5-Methylcytosine in FACS, IF, MeDIP, DB, ICC, ELISA and IHC.

Quick Overview for 5-Methylcytosine (5MeC) antibody (ABIN2668982)

Target: 5-Methylcytosine (5MeC)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (IHC)

Clone: 33D3

Product Details

Purpose: 5-Methylcytosine (5-mC) antibody (mAb) (Clone 33D3)

Purification: Protein A Chromatography

Immunogen: Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.

Isotype: IgG

Application Details

Application Notes: Validated Applications: MeDIP: 1 μg per IP IHC (FFPE): 1:1000 ELISA: 1:10,000 dilution Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.4).

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References

Borghesan, Fusilli, Rappa, Panebianco, Rizzo, Oben, Mazzoccoli, Faulkes, Pata, Agodi, Rezaee, Minogue, Warren, Peterson, Sedivy, Douet, Buschbeck, Cappello, Mazza, Pazienza, Vinciguerra: "DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression." in: Cancer research, Vol. 76, Issue 3, pp. 594-606, (2016) (PubMed).

McPherson, Fullston, Kang, Sandeman, Corbett, Owens, Lane: "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." in: Scientific reports, Vol. 6, pp. 27010, (2016) (PubMed).

Banh, Iorio, Marcotte, Xu, Cojocari, Rahman, Pawling, Zhang, Sinha, Rose, Isasa, Zhang, Wu, Virtanen, Hitomi, Habu, Sidhu, Koizumi, Wilkins, Kislinger, Gygi, Schofield, Dennis, Wouters, Neel: "PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia." in: Nature cell biology, Vol. 18, Issue 7, pp. 803-813, (2016) (PubMed).

Harrison, Cornett, Goldfarb, DaRosa, Li, Yan, Dickson, Guo, Cantu, Kaustov, Brown, Arrowsmith, Erie, Major, Klevit, Krajewski, Kuhlman, Strahl, Rothbart: "Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1." in: eLife, Vol. 5, (2016) (PubMed).

Yang, Ma, Li, Zhang, Wan, Du, Li, Yang, Lu: "Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression." in: Oncology letters, Vol. 12, Issue 4, pp. 2789-2795, (2016) (PubMed).

Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

Xu, Wang, Long, Zhang, Wei, Xing, Gu, Xie: "Stress-mediated p38 activation promotes somatic cell reprogramming." in: Cell research, Vol. 23, Issue 1, pp. 131-41, (2013) (PubMed).

Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." in: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

Habib, Fares, Bourgeois, Bella, Bernardino, Hernandez-Blazquez, de Capoa, Niveleau: "DNA global hypomethylation in EBV-transformed interphase nuclei." in: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, (1999) (PubMed).

Target Details

Target: 5-Methylcytosine (5MeC)

Alternative Name: 5-Methylcytosine

Target Type: Chemical

Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.