POR antibody

Catalog No. ABIN2688654

This Rabbit Polyclonal antibody specifically detects POR in WB and IL. It exhibits reactivity toward Cyanobacteria, Triticum aestivum, Pinus yunnanensis, Phalaenopsis equestris, Pea, Oryza sativa, Nicotiana tabacum, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum) and Arabidopsis thaliana. It has been mentioned in 3+ publications

Quick Overview for POR antibody (ABIN2688654)

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivity: Cyanobacteria, Triticum aestivum, Pinus yunnanensis, Phalaenopsis equestris, Pea, Oryza sativa, Nicotiana tabacum, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POR antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-POR Antibody

Purpose: Anti-POR | Protochlorophilide oxidoreductase

Cross-Reactivity (Details): Arabidopsis thaliana, Cyanobacteria, Horderum vulgare, Nicotiana tabacum, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Pinus yunnanensis, Pisum sativum, Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Serum

Immunogen: Native wheat POR protein isolated from a gel piece

Application Details

Application Notes: 1: 500 (IL), 1 : 2000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-POR antibody (ABIN2688654)

Yuan, Zhang, Zhang, Chen, Yuan, Guo, Lin: "Assembly of NADPH: protochlorophyllide oxidoreductase complex is needed for effective greening of barley seedlings." in: Journal of plant physiology, Vol. 169, Issue 13, pp. 1311-6, (2012) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Wei, Zhou, Hu, Wei, Yang, Huang: "Heterotrimeric G-protein is involved in phytochrome A-mediated cell death of Arabidopsis hypocotyls." in: Cell research, (2008) (PubMed).

Target Details for POR

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Alternative Name: POR

Background: Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.

Molecular Weight: 36-37 kDa (Arabidopsis thaliana)

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome