Asialoglycoprotein Receptor 1 antibody

Catalog No. ABIN2688844

Product Details anti-Asialoglycoprotein Receptor 1 Antibody

Target: Asialoglycoprotein Receptor 1 (ASGR1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Asialoglycoprotein Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 plays a role in serum glycoprotein homeostasis. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. The receptor may also promote hepatic infection by the binding and uptake of various viruses. The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1.

BD Pharmingen™ Purified Mouse Anti-ASGPR 1 - Purified - Clone 8D7 - Isotype Mouse IgG1, κ - Reactivity Hu, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Liver membrane constituents

Clone: 8D7

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry System. Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were fixed with BD Cytofix™ Fixation Buffer and stained with Purified Mouse Anti-ASGPR 1 (pseudo-colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse IgG (Life Technologies), and counter-staining was with DAPI (4',6-Diamidino-2-phenylindole dihydrochloride, pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Asialoglycoprotein Receptor 1 antibody (ABIN2688844)

Touboul, Hannan, Corbineau, Martinez, Martinet, Branchereau, Mainot, Strick-Marchand, Pedersen, Di Santo, Weber, Vallier: "Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 5, pp. 1754-65, (2010) (PubMed).

Shimada, Mizuno, Uesu, Nasu, Okada, Shimomura, Yamamoto, Tsuji, Shiratori: "A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 26, Issue 1, pp. 55-60, (2003) (PubMed).

Mizuno, Yamada, Nagashima: "Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes." in: Gastroenterologia Japonica, Vol. 21, Issue 3, pp. 238-44, (1986) (PubMed).

Target Details for Asialoglycoprotein Receptor 1

Target: Asialoglycoprotein Receptor 1 (ASGR1)

Alternative Name: ASGPR 1 (ASGR1 Products)

Synonyms: ASGR1 antibody, ASGPR antibody, ASGPR1 antibody, CLEC4H1 antibody, HL-1 antibody, Asgr antibody, Asgr-1 antibody, ASGR antibody, RATRHL1 antibody, RHL1 antibody, asialoglycoprotein receptor 1 antibody, LOC721786 antibody, ASGR1 antibody, LOC489461 antibody, Asgr1 antibody, LOC101116157 antibody

Background: Synonyms: Asialoglycoprotein receptor 1, ASGR1, ASGP-R1, ASGPR 1, HL-1, CLEC4H1

Pathways: Thyroid Hormone Synthesis