TNFRSF1B antibody

Catalog No. ABIN2688926

This Rat Monoclonal antibody specifically detects TNFRSF1B in FACS. It exhibits reactivity toward Human. It has been mentioned in 8+ publications

Quick Overview for TNFRSF1B antibody (ABIN2688926)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFRSF1B antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: HTNFR

Product Details anti-TNFRSF1B Antibody

Characteristics: The hTNFR-M1 antibody reacts with the extracellular domain of the 75 kDa transmembrane receptor for the human cytokines, tumor necrosis factor (TNF or TNF-α) and lymphotoxin-alpha (LT-α3, aka, lymphotoxin or TNF-β). This receptor is referred to as the p75 or Type II Tumor Necrosis Factor Receptor (TNFRII) [aka, CD120b]. Human TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. TNFRII is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells. Naive B cells express very low or undetectable levels of TNFRII whereas mature erythrocytes and platelets are uniformly negative for TNFRII expression. The immunogen used to generate the hTNFR-M1 hybridoma was COS- expressed recombinant human TNFRII. Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse™ (Cat. No. 555899) prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 (0.06 μg/10e6 cells, Cat No. 551311) followed by biotinylated goat anti-rat F(ab')2 IgG and streptavidin phycoerythrin (0.015 μg, Cat. No. 554061). Staining with the hTNFR-M1 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes (left panel), monocytes (center panel) and granulocytes (right panel). Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

BD Pharmingen™ Purified Rat Anti-Human CD120b - Purified - Clone hTNFR-M1 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: COS-expressed recombinant human TNFRII

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TNFRSF1B antibody (ABIN2688926)

Aggarwal, Gollapudi, Gupta: "Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 4, pp. 2154-61, (1999) (PubMed).

Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

Gehr, Gentz, Brockhaus, Loetscher, Lesslauer: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, (1992) (PubMed).

Wallach, Engelmann, Nophar, Aderka, Kemper, Hornik, Holtmann, Brakebusch: "Soluble and cell surface receptors for tumor necrosis factor." in: Agents and actions. Supplements, Vol. 35, pp. 51-7, (1992) (PubMed).

Heilig, Mapara, Brockhaus, Krauth, Dörken: "Two types of TNF receptors are expressed on human normal and malignant B lymphocytes." in: Clinical immunology and immunopathology, Vol. 61, Issue 2 Pt 1, pp. 260-7, (1991) (PubMed).

Brockhaus, Schoenfeld, Schlaeger, Hunziker, Lesslauer, Loetscher: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, (1990) (PubMed).

Hohmann, Remy, Brockhaus, van Loon: "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)." in: The Journal of biological chemistry, Vol. 264, Issue 25, pp. 14927-34, (1989) (PubMed).

Munker, DiPersio, Koeffler: "Tumor necrosis factor: receptors on hematopoietic cells." in: Blood, Vol. 70, Issue 6, pp. 1730-4, (1988) (PubMed).

Target Details for TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: CD120b

Background: Synonyms: TNF Receptor Type II

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway