TNFRSF1B antibody (Biotin)

Catalog No. ABIN2688927

There are 8+ publications for this product available. The Rat Monoclonal anti-TNFRSF1B antibody is suitable to detect TNFRSF1B in samples from Human. It has been validated for FACS and ELISA.

Quick Overview for TNFRSF1B antibody (Biotin) (ABIN2688927)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFRSF1B antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: HTNFR

Product Details anti-TNFRSF1B Antibody (Biotin)

Characteristics: The hTNFR-M1 antibody reacts with the extracellular domain of the 75 kDa transmembrane receptor for the human cytokines, tumor necrosis factor (TNF or TNF-α) and lymphotoxin-alpha (LT-α3, aka, lymphotoxin or TNF-β). This receptor is referred to as the p75 or Type II Tumor Necrosis Factor Receptor (TNFRII) [aka, CD120b]. Human TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. TNFRII is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells. Naive B cells express very low or undetectable levels of TNFRII whereas mature erythrocytes and platelets are uniformly negative for TNFRII expression. The immunogen used to generate the hTNFR-M1 hybridoma was COS- expressed recombinant human TNFRII.

BD Pharmingen™ Biotin Rat Anti-Human CD120b - Biotin - Clone hTNFR-M1 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: COS-expressed recombinant human TNFRII

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TNFRSF1B antibody (Biotin) (ABIN2688927)

Aggarwal, Gollapudi, Gupta: "Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 4, pp. 2154-61, (1999) (PubMed).

Gehr, Gentz, Brockhaus, Loetscher, Lesslauer: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, (1992) (PubMed).

Wallach, Engelmann, Nophar, Aderka, Kemper, Hornik, Holtmann, Brakebusch: "Soluble and cell surface receptors for tumor necrosis factor." in: Agents and actions. Supplements, Vol. 35, pp. 51-7, (1992) (PubMed).

Erikstein, Smeland, Blomhoff, Funderud, Prydz, Lesslauer, Espevik: "Independent regulation of 55-kDa and 75-kDa tumor necrosis factor receptors during activation of human peripheral blood B lymphocytes." in: European journal of immunology, Vol. 21, Issue 4, pp. 1033-7, (1991) (PubMed).

Heilig, Mapara, Brockhaus, Krauth, Dörken: "Two types of TNF receptors are expressed on human normal and malignant B lymphocytes." in: Clinical immunology and immunopathology, Vol. 61, Issue 2 Pt 1, pp. 260-7, (1991) (PubMed).

Brockhaus, Schoenfeld, Schlaeger, Hunziker, Lesslauer, Loetscher: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, (1990) (PubMed).

Hohmann, Remy, Brockhaus, van Loon: "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)." in: The Journal of biological chemistry, Vol. 264, Issue 25, pp. 14927-34, (1989) (PubMed).

Munker, DiPersio, Koeffler: "Tumor necrosis factor: receptors on hematopoietic cells." in: Blood, Vol. 70, Issue 6, pp. 1730-4, (1988) (PubMed).

Target Details for TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: CD120b

Background: Synonyms: TNF Receptor Type II

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway