IL3RA antibody

Catalog No. ABIN2688934

Cited in 1 publication. The Mouse Monoclonal anti-IL3RA antibody (Clone 7G3) (ABIN2688934) specifically detects IL3RA in FACS, WB, IP, BR and Neut. The antibody is reactive with Human samples.

Quick Overview for IL3RA antibody (ABIN2688934)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Reagent (BR), Neutralization (Neut)

Clone: 7G3

Product Details anti-IL3RA Antibody

Characteristics: Reacts with human CD123, the 70 kD IL-3 receptor α chain (IL-3Rα), which associates with the 120-140 kD β subunit. The β chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Rα is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors. In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. This antibody has been reported to be useful for immunoprecipitation, western blot and immunofluorescent staining for flow cytometry. At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Human CD123 - Purified - Clone 7G3 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IL-3Ra-transfected cells

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL3RA antibody (ABIN2688934)

Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123

Background: Synonyms: IL-3 Receptor α chain

Pathways: JAK-STAT Signaling