IL4 Receptor antibody

Catalog No. ABIN2688939

Product Details anti-IL4 Receptor Antibody

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The hIL4R-M57 antibody specifically binds to the α subunit (IL-4Rα) of the human Interleukin-4 Receptor complex which is also known as CD124. The human IL-4Rα, also known as B cell stimulatory factor 1 receptor (BSF-1 receptor), is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and non-hematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R complex) or the IL-13 receptor alpha-1 subunit (IL-4Rα/IL-13Rα1, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R complex binds and transduces signals from either IL-4 or IL-13. A truncated form of the IL-4Rα exists in soluble form in biological fluids. In contrast to mice, in humans no distinct mRNA coding for sIL-4R has been described, suggested that human sIL4-R is exclusively produced by proteolytic cleavage of the cell surface receptor. The serum levels of soluble IL-4Rα appear to elevate in pathological situations such as allergy and parasitic infections. Depending on the ratios of IL-4 and sIL-4Rα present in the local milieu, the sIL-4Rα may augment or antagonize the activities of IL-4. The immunogen used to generate the hIL4R-M57 hybridoma was soluble human IL-4R. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of surface CD124 (IL-4Rα) by human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with normal polyclonal human IgG and stained with hIL4R-M57 (0.5 μg/10^6 cells, Cat. No. 551894) followed by biotinylated goat anti-mouse IgG1 and streptavidin phycoerythrin (Cat. No. 554061). Staining with the hIL4R-M57 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The overlapping histograms are displayed for gated CD19 positive lymphocytes. Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

BD Pharmingen™ Purified Mouse Anti-Human CD124 - Purified - Clone hIL4R-M57 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Soluble Human IL-4 Receptor

Clone: HIL4R

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL4 Receptor antibody (ABIN2688939)

Gessner, Röllinghoff: "Biologic functions and signaling of the interleukin-4 receptor complexes." in: Immunobiology, Vol. 201, Issue 3-4, pp. 285-307, (2000) (PubMed).

Jung, Schrader, Hellwig, Enssle, Neumann: "Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases." in: International archives of allergy and immunology, Vol. 119, Issue 1, pp. 23-30, (1999) (PubMed).

Sang, Ouma, John, Whalen, King, Mahmoud, Heinzel: "Increased levels of soluble interleukin-4 receptor in the sera of patients with visceral leishmaniasis." in: The Journal of infectious diseases, Vol. 179, Issue 3, pp. 743-6, (1999) (PubMed).

Jung, Bews, Enssle, Wagner, Neumann, Heusser: "Detection of and discrimination between total and free human interleukin-4 and free soluble interleukin-4 receptor by ELISA." in: Journal of immunological methods, Vol. 217, Issue 1-2, pp. 41-50, (1998) (PubMed).

Nasert, Millner, Enssle, Wahn, Renz: "Differential modulation of T cell functions by soluble IL-4R (s1L-4R) in two cases of severe atopic dermatitis." in: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, Vol. 7, Issue 2, pp. 91-4, (1997) (PubMed).

Hoffman, Castner, Gerhart, Gibson, Rasmussen, March, Weatherbee, Tsang, Gustchina, Schalk-Hihi: "Direct evidence of a heterotrimeric complex of human interleukin-4 with its receptors." in: Protein science : a publication of the Protein Society, Vol. 4, Issue 3, pp. 382-6, (1995) (PubMed).

Zurawski, Chomarat, Djossou, Bidaud, McKenzie, Miossec, Banchereau, Zurawski: "The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor." in: The Journal of biological chemistry, Vol. 270, Issue 23, pp. 13869-78, (1995) (PubMed).

Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

Fanslow, Spriggs, Rauch, Clifford, Macduff, Ziegler, Schooley, Mohler, March, Armitage: "Identification of a distinct low-affinity receptor for human interleukin-4 on pre-B cells." in: Blood, Vol. 81, Issue 11, pp. 2998-3005, (1993) (PubMed).

Mozo, Rivas, Zamorano, Gutierrez: "Differential expression of IL-4 receptors in human T and B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4261-9, (1993) (PubMed).

Zola, Flego, Weedon: "Expression of IL-4 receptor on human T and B lymphocytes." in: Cellular immunology, Vol. 150, Issue 1, pp. 149-58, (1993) (PubMed).

Armitage, Beckmann, Idzerda, Alpert, Fanslow: "Regulation of interleukin 4 receptors on human T cells." in: International immunology, Vol. 2, Issue 11, pp. 1039-45, (1991) (PubMed).

Armitage, Ziegler, Beckmann, Idzerda, Park, Fanslow: "Expression of receptors for interleukin 4 and interleukin 7 on human T cells." in: Advances in experimental medicine and biology, Vol. 292, pp. 121-30, (1991) (PubMed).

Law, Armitage, Villablanca, LeBien: "Expression of interleukin-4 receptors on early human B-lineage cells." in: Blood, Vol. 78, Issue 3, pp. 703-10, (1991) (PubMed).

Idzerda, March, Mosley, Lyman, Vanden Bos, Gimpel, Din, Grabstein, Widmer, Park: "Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily." in: The Journal of experimental medicine, Vol. 171, Issue 3, pp. 861-73, (1990) (PubMed).

Zuber, Galizzi, Harada, Durand, Banchereau: "Interleukin-4 receptors on human blood mononuclear cells." in: Cellular immunology, Vol. 129, Issue 2, pp. 329-40, (1990) (PubMed).

Zuber, Galizzi, Vallé, Harada, Howard, Banchereau: "Interleukin 4 receptors on normal human B lymphocytes: characterization and regulation." in: European journal of immunology, Vol. 20, Issue 3, pp. 551-5, (1990) (PubMed).

Ohara, Paul: "Receptors for B-cell stimulatory factor-1 expressed on cells of haematopoietic lineage." in: Nature, Vol. 325, Issue 6104, pp. 537-40, (1987) (PubMed).

Park, Friend, Sassenfeld, Urdal: "Characterization of the human B cell stimulatory factor 1 receptor." in: The Journal of experimental medicine, Vol. 166, Issue 2, pp. 476-88, (1987) (PubMed).

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: CD124 (IL4R Products)

Synonyms: IL4R antibody, il-4ra antibody, zgc:113063 antibody, CD124 antibody, IL-4RA antibody, IL4RA antibody, Il4ra antibody, IL4 antibody, Il4r antibody, interleukin 4 receptor antibody, interleukin 4 receptor, tandem duplicate 1 antibody, interleukin 4 receptor, alpha antibody, IL4R antibody, il4r.1 antibody, Il4r antibody, Il4ra antibody

Background: Synonyms: IL-4 Receptor α Chain

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process