FCGR3A antibody (Biotin)

Catalog No. ABIN2689011

There are 7+ publications for this product available. The Mouse Monoclonal anti-FCGR3A antibody is suitable to detect FCGR3A in samples from Rat. It has been validated for FACS.

Quick Overview for FCGR3A antibody (Biotin) (ABIN2689011)

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR3A antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 10-78

Product Details anti-FCGR3A Antibody (Biotin)

Characteristics: The 10/78 antibody reacts with NKR-P1A, a 60- kDa homodimer expressed on all natural killer (NK) cells and a small subset of T lymphocytes. The 10/78 antibody competes with the previously described 3.2.3 antibody for binding to the antigen. NKR-P1A is a type-II integral membrane protein with an extracellular C-type lectin domain, which is an NK cell-activating receptor specific for tumor target cells. Many rat dendritic cells have been shown to express NKR-P1A, and a subpopulation of these cells has cytotoxic activity. NKR-P1A has also been detected at low levels on peripheral blood monocytes, and its expression is upregulated in IFN-γ-activated monocytes, specifically in a subpopulation of large monocytes with phagocytic capacity. Furthermore, activated peripheral blood neutrophils may express a low level of NKR-P1A. In the mouse and rat, three members of the NKR-P1 gene family have been identified, but in the human gene family, a single NKR-P1 homologue has been discovered and designated Cd161.

BD Pharmingen™ Biotin Mouse Anti-Rat CD161a - Biotin - Clone 10/78 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-FCGR3A antibody (Biotin) (ABIN2689011)

Trinité, Voisine, Yagita, Josien: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, (2000) (PubMed).

Scriba, Schneider, Grau, van der Meide, Steiniger: "Rat monocytes up-regulate NKR-P1A and down-modulate CD4 and CD43 during activation in vivo: monocyte subpopulations in normal and IFN-gamma-treated rats." in: Journal of leukocyte biology, Vol. 62, Issue 6, pp. 741-52, (1997) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." in: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Lanier: "Natural killer cells: from no receptors to too many." in: Immunity, Vol. 6, Issue 4, pp. 371-8, (1997) (PubMed).

Ryan, Niemi, Nakamura, Seaman: "NKR-P1A is a target-specific receptor that activates natural killer cell cytotoxicity." in: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1911-5, (1995) (PubMed).

Bezouska, Vlahas, Horváth, Jinochová, Fiserová, Giorda, Chambers, Feizi, Pospísil: "Rat natural killer cell antigen, NKR-P1, related to C-type animal lectins is a carbohydrate-binding protein." in: The Journal of biological chemistry, Vol. 269, Issue 24, pp. 16945-52, (1994) (PubMed).

Bezouska, Yuen, OBrien, Childs, Chai, Lawson, Drbal, Fiserová, Pospísil, Feizi: "Oligosaccharide ligands for NKR-P1 protein activate NK cells and cytotoxicity." in: Nature, Vol. 372, Issue 6502, pp. 150-7, (1994) (PubMed).

Target Details for FCGR3A

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Alternative Name: CD161a

Background: Synonyms: NKR-P1A