CD19 antibody

Catalog No. ABIN2689047

There are 4+ publications for this product available. The Mouse Monoclonal anti-CD19 antibody is suitable to detect CD19 in samples from Human. It has been validated for FACS, IHC (fro) and FM.

Quick Overview for CD19 antibody (ABIN2689047)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence Microscopy (FM)

Clone: HIB19

Product Details anti-CD19 Antibody

Characteristics: Reacts with the 95 kDa type I transmembrane glycoprotein expressed during all stages of B-cell differentiation and maturation, except on plasma cells. CD19 is also present on follicular dendritic cells. It is not found on T cells or on normal granulocytes. CD19 plays a role in regulation of B-cell proliferation. It associates with the complement receptor 2 (CD21), TAPA-1 (CD81), Leu 13, and/or MHC class II to form a signal transduction complex on the surface of B cells. Anti-CD19 clone HIB19 partially blocks clone B43, another C19 mAb. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Purified Mouse Anti-Human CD19 - Purified - Clone HIB19 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD19 antibody (ABIN2689047)

Bradbury, Goldmacher, Tedder: "The CD19 signal transduction complex of B lymphocytes. Deletion of the CD19 cytoplasmic domain alters signal transduction but not complex formation with TAPA-1 and Leu 13." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 6, pp. 2915-27, (1993) (PubMed).

Favaloro, Moraitis, Koutts, Exner, Bradstock: "Endothelial cells and normal circulating haemopoietic cells share a number of surface antigens." in: Thrombosis and haemostasis, Vol. 61, Issue 2, pp. 217-24, (1989) (PubMed).

Uckun, Muraguchi, Ledbetter, Kishimoto, OBrien, Roloff, Gajl-Peczalska, Provisor, Koller: "Biphenotypic leukemic lymphocyte precursors in CD2+CD19+ acute lymphoblastic leukemia and their putative normal counterparts in human fetal hematopoietic tissues." in: Blood, Vol. 73, Issue 4, pp. 1000-15, (1989) (PubMed).

Nadler, Anderson, Marti, Bates, Park, Daley, Schlossman: "B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 244-50, (1983) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway