CD19 antibody (Biotin)

Catalog No. ABIN2689050

Product Details anti-CD19 Antibody (Biotin)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Brand: BD Pharmingen™

Characteristics: The 1D3 antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 95 kDa transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 Molecule associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cells in the bone marrow and periphery. B-1 lineage B cells, also known as CD5+ B cells, are drastically reduced or absent in CD19-deficient mice. Increased levels of CD19 expression correlate with increased frequencies of peritonal and splenic B-1 cells and reduced numbers of conventional B lymphocytes in the periphery. CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063) in the absence (left panel) or presence (right panel) of biotin-conjugated 1D3 mAb followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse CD19 - Biotin - Clone 1D3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CD19 Transfected Cell Line

Clone: 1D3

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD19 antibody (Biotin) (ABIN2689050)

Gommerman, Oh, Zhou, Tedder, Maurer, Galli, Carroll: "A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 6915-21, (2000) (PubMed).

Sato, Jansen, Tedder: "CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 13158-62, (1998) (PubMed).

Inaoki, Sato, Weintraub, Goodnow, Tedder: "CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes." in: The Journal of experimental medicine, Vol. 186, Issue 11, pp. 1923-31, (1997) (PubMed).

Sato, Miller, Howard, Tedder: "Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 7, pp. 3278-87, (1997) (PubMed).

Sato, Steeber, Jansen, Tedder: "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4662-9, (1997) (PubMed).

Krop, Shaffer, Fearon, Schlissel: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, (1996) (PubMed).

Krop, de Fougerolles, Hardy, Allison, Schlissel, Fearon: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).

Sato, Ono, Steeber, Pisetsky, Tedder: "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4371-8, (1996) (PubMed).

Engel, Zhou, Ord, Sato, Koller, Tedder: "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." in: Immunity, Vol. 3, Issue 1, pp. 39-50, (1995) (PubMed).

Rickert, Rajewsky, Roes: "Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice." in: Nature, Vol. 376, Issue 6538, pp. 352-5, (1995) (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).

Fearon: "The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes." in: Current opinion in immunology, Vol. 5, Issue 3, pp. 341-8, (1993) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Synonyms: B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway