ITGB1 antibody

Catalog No. ABIN2689155

The Rat Monoclonal anti-ITGB1 antibody has been validated for WB, FACS, ELISA, IP and Func. It is suitable to detect ITGB1 in samples from Human. There are 4+ publications available.

Quick Overview for ITGB1 antibody (ABIN2689155)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Clone: Mab 13

Product Details anti-ITGB1 Antibody

Brand: BD Pharmingen™

Characteristics: The Mab 13 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The family of β1 integrins includes receptors for vascular cell adhesion molecule 1 (VCAM-1), extracellular matrix (ECM) components such as collagen (COL), fibronectin (FN), laminin (LM) and vitronectin (VN), and pathogens. The β1 subunit is widely expressed by T and B cells, dendritic cells, NK cells, monocytes and macrophages, granulocytes, platelets, endothelial and epithelial cells, and stem cells. Flow cytometric analysis of CD29 expression on human peripheral blood lymphocytes. Whole blood was stained with Purified Rat Anti-Human CD29 antibody (Cat. No. 552828, solid line histogram) or with Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927/555841, dashed line histogram), followed by FITC Goat Anti-Rat Ig (Cat. No 554016). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

BD Pharmingen™ Purified Rat Anti-Human CD29 - Purified - Clone Mab 13 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified Fibronectin Receptor from Human Placenta

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGB1 antibody (ABIN2689155)

Bohnsack: "CD11/CD18-independent neutrophil adherence to laminin is mediated by the integrin VLA-6." in: Blood, Vol. 79, Issue 6, pp. 1545-52, (1992) (PubMed).

Chan, Elices, Murphy, Hemler: "Adhesion to vascular cell adhesion molecule 1 and fibronectin. Comparison of alpha 4 beta 1 (VLA-4) and alpha 4 beta 7 on the human B cell line JY." in: The Journal of biological chemistry, Vol. 267, Issue 12, pp. 8366-70, (1992) (PubMed).

Hertle, Adams, Watt: "Integrin expression during human epidermal development in vivo and in vitro." in: Development (Cambridge, England), Vol. 112, Issue 1, pp. 193-206, (1992) (PubMed).

Teixidó, Hemler, Greenberger, Anklesaria: "Role of beta 1 and beta 2 integrins in the adhesion of human CD34hi stem cells to bone marrow stroma." in: The Journal of clinical investigation, Vol. 90, Issue 2, pp. 358-67, (1992) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29

Background: Synonyms: ITGB1, Integrin beta-1, Integrin β1, FNRB, GPIIA, ITB1, MDF2, MSK12, VLA-4β

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome