ITGB1 antibody

Catalog No. ABIN2689157

This Armenian Hamster Monoclonal antibody specifically detects ITGB1 in FACS, IHC (fro) and IHC (f). It exhibits reactivity toward Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for ITGB1 antibody (ABIN2689157)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HM beta1

Product Details anti-ITGB1 Antibody

Brand: BD Pharmingen™

Characteristics: The HM β1-1 monoclonal antibody specifically binds to the 130- kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin-α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. It also associates with the integrin α7 α8, and α9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes. This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes. Source of the immunogen was purified mouse VLA-4 (α4β1, CD49d/CD29). Immunohistochemical staining of CD29 positive cells. Frozen sections of normal mouse spleen were stained with Purified Hamster Anti-Mouse CD29 (Cat. No. 550530) antibody. Endothelial cells of the central arteriole and splenic sinuses can be identified by the brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Hamster Anti-Mouse CD29 - Purified - Clone HM β1-1 - Isotype Armenian Hamster IgG2, λ1 - Reactivity Ms, Rat - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified mouse VLA-4

Isotype: IgG2 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 15.625 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGB1 antibody (ABIN2689157)

Noto, Kato, Okumura, Yagita: "Identification and functional characterization of mouse CD29 with a mAb." in: International immunology, Vol. 7, Issue 5, pp. 835-42, (1995) (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29

Background: Synonyms: Itgb1, Integrin beta-1, Fnrb, gpIIa, VLA-4 subunit beta

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome