ITGB1 antibody (Biotin)

Catalog No. ABIN2689159

This anti-ITGB1 antibody is a Armenian Hamster Monoclonal antibody detecting ITGB1 in FACS. Suitable for Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for ITGB1 antibody (Biotin) (ABIN2689159)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: Ha2-5

Product Details anti-ITGB1 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The Ha2/5 antibody reacts with the 130 kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αV (CD51), it forms αVβ1 integrin. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. The Ha2/5 hamster anti-rat CD29 monoclonal antibody cross-reacts with mouse thymocytes, splenocytes, and peripheral lymph node leukocytes. The Ha2/5 antibody blocks in vitro adhesion of CD29-expressing cells to collagen.

BD Pharmingen™ Biotin Hamster Anti-Rat CD29 - Biotin - Clone Ha2/5 - Isotype Armenian Hamster IgM, κ - Reactivity Rat, Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat glomerular epithelial cells

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-ITGB1 antibody (Biotin) (ABIN2689159)

Mendrick, Kelly: "Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 690-702, (1994) (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29

Background: Synonyms: Integrin β1 chain

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome