CD4 antibody

Catalog No. ABIN2689224

The Rat Monoclonal anti-CD4 antibody has been validated for FACS, IP, IHC (fro), IHC (f) and IHC (zinc). It is suitable to detect CD4 in samples from Mouse. There are 13+ publications available.

Quick Overview for CD4 antibody (ABIN2689224)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: RM4

Product Details anti-CD4 Antibody

Brand: BD Pharmingen™

Characteristics: The RM4-5 clone has been reported to react with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells. CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone RM4-5 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Thymocytes (BALB/c)

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689224)

Martín, del Hoyo, Anjuère, Ruiz, Arias, Marín, Ardavín: "Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." in: Blood, Vol. 96, Issue 7, pp. 2511-9, (2000) (PubMed).

Shevach: "Regulatory T cells in autoimmmunity*." in: Annual review of immunology, Vol. 18, pp. 423-49, (2000) (PubMed).

Allman, Li, Hardy: "Commitment to the B lymphoid lineage occurs before DH-JH recombination." in: The Journal of experimental medicine, Vol. 189, Issue 4, pp. 735-40, (1999) (PubMed).

Bosselut, Zhang, Ashe, Kopacz, Samelson, Singer: "Association of the adaptor molecule LAT with CD4 and CD8 coreceptors identifies a new coreceptor function in T cell receptor signal transduction." in: The Journal of experimental medicine, Vol. 190, Issue 10, pp. 1517-26, (1999) (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

Guo, Watanabe, Mori, Mori: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, (1995) (PubMed).

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, (1994) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Wineman, Gilmore, Gritzmacher, Torbett, Müller-Sieburg: "CD4 is expressed on murine pluripotent hematopoietic stem cells." in: Blood, Vol. 80, Issue 7, pp. 1717-24, (1992) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, (1991) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, (1989) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Synonyms: L3T4

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events