CD4 antibody

Catalog No. ABIN2689225

This anti-CD4 antibody is a Rat Monoclonal antibody detecting CD4 in FACS, IP, IHC (fro), BR, IHC (f) and IHC (zinc). Suitable for Mouse. This Primary Antibody has been cited in 15+ publications.

Quick Overview for CD4 antibody (ABIN2689225)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: H129-19

Product Details anti-CD4 Antibody

Brand: BD Pharmingen™

Characteristics: The H129.19 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine lck. H129.19 mAb blocks binding of the anti-mouse CD4 mAbs Gk1.5 (Cat. No. 557307/553729) and RM4-5 (Cat. No. 553046/553047), but not RM4-4 (Cat. No. 553055) antibody. mAb H129.19 inhibits various responses of T helper cells to antigenic or mitogenic stimuli. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone H129.19 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: A.TH mouse CTL clone A15.1.17

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD4 antibody (ABIN2689225)

Martín, del Hoyo, Anjuère, Ruiz, Arias, Marín, Ardavín: "Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." in: Blood, Vol. 96, Issue 7, pp. 2511-9, (2000) (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

Guo, Watanabe, Mori, Mori: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, (1995) (PubMed).

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, (1994) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Wineman, Gilmore, Gritzmacher, Torbett, Müller-Sieburg: "CD4 is expressed on murine pluripotent hematopoietic stem cells." in: Blood, Vol. 80, Issue 7, pp. 1717-24, (1992) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, (1991) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, (1989) (PubMed).

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, (1989) (PubMed).

Regnier-Vigouroux, Blanc, Pont, Marchetto, Pierres: "Accessory molecules and T cell activation. I. Antigen receptor avidity differentially influences T cell sensitivity to inhibition by monoclonal antibodies to LFA-1 and L3T4." in: European journal of immunology, Vol. 16, Issue 11, pp. 1385-90, (1987) (PubMed).

Pont, Régnier-Vigouroux, Marchetto, Pierres: "Accessory molecules and T cell activation. II. Antibody binding to L3T4a inhibits Ia-independent mouse T cell proliferation." in: European journal of immunology, Vol. 17, Issue 3, pp. 429-32, (1987) (PubMed).

Naquet, Malissen, Bekkhoucha, Pont, Pierres, Hood, Pierres: "L3T4 but not LFA-1 participates in antigen presentation by Ak-positive L-cell transformants." in: Immunogenetics, Vol. 22, Issue 3, pp. 247-56, (1985) (PubMed).

Pierres, Naquet, Van Agthoven, Bekkhoucha, Denizot, Mishal, Schmitt-Verhulst, Pierres et al.: "A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 6, pp. 2775-82, (1984) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Synonyms: L3T4

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events