CD43 antibody

Catalog No. ABIN2689256

Product Details anti-CD43 Antibody

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The S7 antibody reacts with the 115 kDa glycoform of CD43 (Ly-48, Leukosialin) which is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. Immunohistochemical staining of a mouse spleen. A citrate pre-treated, formalin-fixed paraffin-embedded section of a normal mouse spleen was stained with 0.3125 μg/mL of the purified rat anti-mouse CD43 antibody (clone S7) (20X magnification).

BD Pharmingen™ Purified Rat Anti-Mouse CD43 - Purified - Clone S7 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Plasmacytoma MOPC-315

Clone: S7

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 7.8 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD43 antibody (ABIN2689256)

Stockton, Cheng, Manjunath, Ardman, von Andrian: "Negative regulation of T cell homing by CD43." in: Immunity, Vol. 8, Issue 3, pp. 373-81, (1998) (PubMed).

Thurman, Walker, Jayaraman, Manjunath, Ardman, Green: "Regulation of in vitro and in vivo T cell activation by CD43." in: International immunology, Vol. 10, Issue 5, pp. 691-701, (1998) (PubMed).

Rolink, ten Boekel, Melchers, Fearon, Krop, Andersson: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, (1996) (PubMed).

Wells, Kantor, Stall: "CD43 (S7) expression identifies peripheral B cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 12, pp. 5503-15, (1995) (PubMed).

Moore, Bennett, Kumar: "Transplantable NK cell progenitors in murine bone marrow." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1653-63, (1995) (PubMed).

Jones, Federsppiel, Ellies, Williams, Burgener, Duronio, Smith, Takei, Ziltener: "Characterization of the activation-associated isoform of CD43 on murine T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3426-39, (1994) (PubMed).

Moore, Huang, Terstappen, Bennett, Kumar: "Expression of CD43 on murine and human pluripotent hematopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4978-87, (1994) (PubMed).

Hardy, Hayakawa: "Generation of Ly-1 B cells from developmentally distinct precursors. Enrichment by stromal-cell culture or cell sorting." in: Annals of the New York Academy of Sciences, Vol. 651, pp. 99-111, (1992) (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).

Gulley, Ogata, Thorson, Dailey, Kemp: "Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3751-7, (1988) (PubMed).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 (SPN Products)

Synonyms: CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody

Background: Synonyms: Ly-48, Leukosialin

Pathways: Regulation of Leukocyte Mediated Immunity