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CD45RB antibody (Biotin)

CD45RB Reactivity: Mouse FACS, IF Host: Rat Monoclonal 16A Biotin
Catalog No. ABIN2689290
  • Target See all CD45RB Antibodies
    CD45RB
    Reactivity
    • 58
    • 20
    • 7
    • 4
    • 3
    • 3
    • 2
    Mouse
    Host
    • 47
    • 20
    • 11
    Rat
    Clonality
    • 78
    Monoclonal
    Conjugate
    • 21
    • 8
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45RB antibody is conjugated to Biotin
    Application
    • 75
    • 40
    • 37
    • 24
    • 18
    • 16
    • 7
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Brand
    BD Pharmingen™
    Characteristics
    The 16A antibody reacts with an exon B-dependent epitope of CD45 lycoprotein, which is found at high density on peripheral B cells, T cytotoxic/suppressor cells, subset of T helper cells, and most thymocytes and at low density on macrophages and dendritic cells.The level of CD45RB expression appears to decrease as T lymphocytes progress from naive to memory cells. In addition, subpopulations of CD4+ T cells which express high and low levels of D45RB have different ytokine secretion profiles and mediate distinct immunological functions. CD25+ D4+ regulatory T (Treg) lymphocytes which control intestinal inflammation and autoimmunity express low levels of D45RB. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

    BD Pharmingen™ Biotin Rat Anti-Mouse CD45RB - Biotin - Clone 16A - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Clone
    16A
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CD45RB Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

    Ernst, Weigle, Noonan, McQuitty, Hobbs: "The age-associated increase in IFN-gamma synthesis by mouse CD8+ T cells correlates with shifts in the frequencies of cell subsets defined by membrane CD44, CD45RB, 3G11, and MEL-14 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 2, pp. 575-87, (1993) (PubMed).

    Inoue, Asano, Matsuoka, Furutani-Seiki, Aizawa, Nishimura, Shirai, Tada: "Distinction of mouse CD8+ suppressor effector T cell clones from cytotoxic T cell clones by cytokine production and CD45 isoforms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 6, pp. 2121-8, (1993) (PubMed).

    Rogers, Pilapil, Hayakawa, Romain, Parker: "CD45 alternative exon expression in murine and human CD4+ T cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 4054-65, (1992) (PubMed).

    Hathcock, Laszlo, Dickler, Sharrow, Johnson, Trowbridge, Hodes: "Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 1, pp. 19-28, (1992) (PubMed).

    Dianzani, Luqman, Rojo, Yagi, Baron, Woods, Janeway, Bottomly: "Molecular associations on the T cell surface correlate with immunological memory." in: European journal of immunology, Vol. 20, Issue 10, pp. 2249-57, (1990) (PubMed).

    Bottomly, Luqman, Greenbaum, Carding, West, Pasqualini, Murphy: "A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines." in: European journal of immunology, Vol. 19, Issue 4, pp. 617-23, (1989) (PubMed).

    Johnson, Greenbaum, Bottomly, Trowbridge: "Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies." in: The Journal of experimental medicine, Vol. 169, Issue 3, pp. 1179-84, (1989) (PubMed).

  • Target
    CD45RB
    Alternative Name
    CD45RB (CD45RB Products)
    Synonyms
    B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody
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