ITGA2 antibody

Catalog No. ABIN2689301

Product Details anti-ITGA2 Antibody

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Brand: BD Pharmingen™

Characteristics: The rat anti-mouse CD49b antibody (clone DX5) reacts with the integrin α2 chain (CD49b), a 150 kDa transmembrane glycoprotein that non-covalently associates with CD29 (integrin β1), to form the integrin α2β1 complex known as VLA-2. The rat anti-mouse CD49b antibody (clone DX5) has been reported to identify the majority of NK cells and a small T-cell subpopulation in most strains (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, DBA/1, DBA/2, SJL, SWR, 129/J, but not NOD). It has also been observed that this antibody recognizes platelets, which express high levels of CD49b. Multi-parameter flow cytometric analysis has demonstrated that most lymphocytes which express NK-1.1 (NKR-P1B and NKR-P1C), as detectable by mouse anti-mouse NK-1.1 antibody (clone PK136), also express the DX5 antigen. Small DX5+ NK-1.1- and DX5- NK-1.1+ subsets are found, especially among the CD3-positive population. Some CD49b+ NK cells have been reported to gradually lose reactivity with the rat anti-mouse CD49b antibody (clone DX5) when cultured in the presence of recombinant human IL-2, and the resulting DX5- cells have weakened cytotoxic activity when compared to the remaining DX5+ cells, indicating that the DX5 antibody distinguishes functional subsets of NK cells. No activation or blocking activity of the rat anti-mouse antibody (clone DX5) has been observed while staining of splenic NK cells by this antibody reportedly can be blocked by hamster anti-mouse CD49b antibody (clone HMα2). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of NK cells with monoclonal antibodies. Freshly isolated splenocytes from a C57BL/6 mouse were simultaneously incubated with PE mouse anti-mouse NK-1.1 antibody (NKR-P1B and NKR-P1C) (clone PK136) (MN 557391, both panels), and purified rat anti-mouse CD49b antibody (clone DX5) (right panel), followed by a FITC mouse anti-rat IgM antibody (clone G53-238) (Cat. No. 553887, both panels). Flow cytometry was performed on a BD FACScan™ instrument . Page 1 of 2553855 Rev. 11

BD Pharmingen™ Purified Rat Anti-Mouse CD49b - Purified - Clone DX5 - Isotype Rat IgM, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse (C57BL/6) NK1.1+ cells propagated with rIL-2

Clone: DX5

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGA2 antibody (ABIN2689301)

Arase, Saito, Phillips, Lanier: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).

Sepulveda, Cerwenka, Morgan, Dutton: "CD28, IL-2-independent costimulatory pathways for CD8 T lymphocyte activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 3, pp. 1133-42, (1999) (PubMed).

Ortaldo, Winkler-Pickett, Mason, Mason: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).

Moore, von Freeden-Jeffry, Murray, Zlotnik: "Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2366-73, (1996) (PubMed).

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: CD49b (ITGA2 Products)

Synonyms: BR antibody, CD49B antibody, GPIa antibody, HPA-5 antibody, VLA-2 antibody, VLAA2 antibody, DX5 antibody, integrin subunit alpha 2 antibody, integrin alpha 2 antibody, ITGA2 antibody, Itga2 antibody

Background: Synonyms: Pan-NK cells

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex